Lgr5-EGFP-IRES- creERT2 knock-in-möss matades dieter innehållande n-6 PUFA (kontroll), n-3 PUFA, n-6 PUFA + curcumin eller n-3 PUFA +

Although small and large intestines possess seemingly similar Wnt-driven leucine-rich repeat-containing G protein–coupled receptor 5 (Lgr5)+ adult epithelial stem cells, we report here that the two organs exhibit distinct mechanisms of tissue response to ionizing radiation. Employing Lgr5-lacZ transgenic mice and Lgr5 in situ hybridization, we found colonic epithelial stem cells (CESC

Involvement Lgr5 RSCs in rectal epithelial regeneration was examined by lineage tracing assay. To this end, we first established a sorting strategy that would enable the isolation of pure populations of Lgr5 + E10.5-E12.5 hepatoblasts using the Lgr5-EGFP-IRES-creERT2 mouse line, where the eGFP reporter is knocked-in into the Lgr5 locus (Barker et al., 2007), combined with co-staining using an anti-Liv2 antibody, which specifically labels E9.5-E13.5 liver progenitors (Nierhoff et al Se hela listan på blog.csdn.net Lgr5-EGFP-IRES-creERT2基因敲入工具鼠索取资料欢迎您在此索取产品资料，联系订购产品，提交后您的需求信息将立即自动发送到供应商的E-mail邮箱，同时也将抄送一封到您的邮箱，感谢您的惠顾！ with WT Lgr5-EGFP-ires-creERT2 mice and by multiple generation selective breedings obtaining mice that were WT, CD44 /,or TLR4 / and heterozygous for the Lgr5-EGFP-ires-creERT2 re-porter. Mice were maintained on a 12-h:12-h light/dark schedule in a temperature-controlled speciﬁc pathogen-free facility and fed stan-dard laboratory mouse chow. The Lgr5 EGFP-Ires-CreERT2 knock-in mice (Barker et al., 2007) and Rosa26-LacZ reporter mice were obtained from The Jackson Laboratory.

ZERO BIAS - scores, article reviews, protocol conditions and more The Jackson Laboratory lgr5 egfp ires creert2 Lgr5 Egfp Ires Creert2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Lgr5-cre, Lgr5CREER, Lgr5-creERT, Lgr5-CreERT2-IRES-EGFP, Lgr5-EGFP, Lgr5 EGFP-IRES-creERT2, Lgr5-EGFP-IRES-creERT2, Lgr5 tm1(cre/ESR1)Cle: Gene: Lgr5 Location: Chr10:115450314-115587780 bp, - strand Genetic Position: Chr10, 63.51 cM The Jackson Laboratory lgr5 egfp ires creert2 Lgr5 Egfp Ires Creert2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 89/100, based on 50 PubMed citations.

While homozygous mice are not viable, heterozygous Lgr5-EGFP-IRES-CreERT2 mice are viable and fertile; harboring a Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 (Gpr49) gene function and expresses EGFP and CreERT2 fusion protein from the Lgr5 promoter/enhancer elements.

Lgr5‐EGFP‐ires‐CreERT2 × Rosa26‐Tomato mice at postnatal day 40 and 41 (P40, P41) were divided into treated and untreated groups (n = 3). One week postinduction with Tam, the mice were sacrificed and the tongues were dissected out.

Only Lgr5 and Ascl2 were enriched in the GFPhi stem cells of the colon. Expression of Bmi1, another putative N et al. Biol Res P 6 13 G007‑LKeatment repressed lineage tracing from˜ LGR5+ intestinal stem cells Lgr5-EGFP-Ires-CreERT2;R26R-Confettitdoublettrans- genictmicet adult Lgr5-EGFP-ires-CreERT2/Rosa four-color mice.

Name * Company * Phone No. * Email * Research Field. Remark. Submit. Contact us Gene-editing proposal Back to top Heterozygous mice harbor an Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function and expresses EGFP and CreERT2 fusion protein. When these mice mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Lgr5-expressing cells of the offspring.
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Confocal. analyses of the clonal output were performed at 14 days, 2 months, 6 months, and 12 months after the ﬁnal tamoxifen.

Biol Res P 6 13 G007‑LKeatment repressed lineage tracing from˜ LGR5+ intestinal stem cells Lgr5-EGFP-Ires-CreERT2;R26R-Confettitdoublettrans- genictmicet adult Lgr5-EGFP-ires-CreERT2/Rosa four-color mice. Confocal. analyses of the clonal output were performed at 14 days, 2 months, 6 months, and 12 months after the ﬁnal tamoxifen. Long-term culture of adult progenitor cells in 3D is a recently emerging technology that inhabits the space between 2D cell lines and organ slice culture.
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Intestinal Crypt Homeostasis Results from Neutral Competition between Symmetrically Dividing Lgr5 Stem Cells Hugo J. Snippert,1 Laurens G. van der Flier,1 Toshiro Sato,1 Johan H. van Es,1 Maaike van den Born,1 Carla Kroon-Veenboer, 1Nick Barker, Allon M. Klein,2, 3Jacco van Rheenen, 1Benjamin D. Simons, and Hans Clevers ,* 1Hubrecht Institute, KNAW and University Medical Center Utrecht

H pylori colonize and manipulate the progenitor and stem cell compartments, which alters turnover kinetics and glandular hyperplasia. Bacterial ability to alter the stem cells has important implications for gastrointestinal stem cell biology and H pylori-induced gastric pathology. By using the knock-in allele Lgr5 EGFP-Ires-CreERT2, we have shown that the cell surface receptor Lgr5 (leucine-rich repeat–containing heterotrimeric guanine nucleotide–binding protein–coupled receptor 5) marks normal tissue stem cells in stomach, small intestine, colon, and hair follicles . 2012-09-27 · Lgr5-EGFP-Ires-CreERT2, Lgr5-lacZ, and Rosa26-4color mice were generated as described previously (Barker et al., 2007).

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To assess whether all epithelial tumours of the gastrointestinal tract are derived from LGR5-positive stem cells, we crossed Lgr5-EGFP-IRES-creER(T2) mice, which express EGFP and Cre recombinase driven by the Lgr5 promoter, with CEA424-SV40-TAg mice, which develop pyloric neuroendocrine carcinomas of epithelial origin.

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Using Villin-creERT2;Tnks1-/-;Tnks2fl/fl mice, which harbored conventional Tnks1 of Ctrl (Lgr5-EGFP-IRES-creERT2) or DKO-Lgr5 (Lgr5-EGFP-IRES- creERT2

Bioz Stars score: 89/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more The Jackson Laboratory lgr5 egfp ires creert2 Lgr5 Egfp Ires Creert2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.

2021-04-06 · in the case of intestinal organoids from Lgr5-EGFP-IRES-CreERT2 transgenic mice, in vitro expansion of Lgr5 expression is limited in a culture condition supplemented with essential proteins; NHE8 deficiency resulted in increased Lgr5 expression in the colon. LGR5 promotes cancer stem cell traits and chemoresistance in cervical cancer. 2012-09-27 · Lgr5-EGFP-Ires-CreERT2, Lgr5-lacZ, and Rosa26-4color mice were generated as described previously (Barker et al., 2007). Rosa26RlacZ mice were obtained from Jackson Labs. All animal experiments were approved by the Animal Experimentation Committee of the Royal Dutch Academy of Science and the Institutional Animal Care and Use Committee of Singapore.